Calpain-3 stability following delays in freezing skeletal muscle biopsy samples-stablishing an optimal time frame for accurate interpretation

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Calpain-3 is a 94 kDa protein which autolyses in the presence of slight increases in Ca2+, to give 60, 58 and 55 kDa autolytic products. Homeostasis of Ca2+ during excitation contraction coupling is highly regulated. Calpain-3 may be involved in the proteolysis of some of the proteins involved with this process. Diagnostic muscle samples can experience delays in delivery to the laboratory, potentially leading to artefactual loss of enzymatic protein and incorrect interpretation of Calpain-3. We examined the implications of delays in freezing muscle for diagnosis of LGMD2A.

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