LIMB-GIRDLE MUSCULAR DYSTROPHY I

0

Mouse and rat muscle cell lines can not completely replace research made on human cells. The human rhabdomyosarcoma cell line TE671 has been used extensively to study different aspects of muscle biology. TE671 bears a mutation in the k-RAS gene that produces an increase in Ras activity, a key element in the signal transduction pathway MAPK / ERK. However, the ability of TE671 to differentiate and form myotubes has not been fully explored. In the present study, we examined muscle differentiation when we specifically stopped proliferation of human TE671 (WT-TE671) cells by using 1,4-diamino-2, 3-dicyano-1,4-bis[2-aminophenylthio] butadiene (U0126), a MAP kinase inhibitor.

Read More...

Leave A Reply