Multimodal Single-Cell Analysis Reveals Physiological Maturation in the Developing Human Neocortex

By Simone Mayer, Jiadong Chen, Dmitry Velmeshev, Andreas Mayer, Ugomma C. Eze, Aparna Bhaduri, Carlos E. Cunha, Diane Jung, Arpana Arjun, Emmy Li, Beatriz Alvarado, Shaohui Wang, Nils Lovegren, Michael L. Gonzales, Lukasz Szpankowski, Anne Leyrat, Jay A.A. West, Georgia Panagiotakos, Arturo Alvarez-Buylla, Mercedes F. Paredes, Tomasz J. Nowakowski, Alex A. Pollen, Arnold R. Kriegstein On Feb 12, 2019

Mayer et al. develop a microfluidics-based approach that links calcium imaging and single-cell transcriptomics to study cellular responses to neurotransmitters in the developing human neocortex. They reveal dynamically changing response profiles as progenitor cells differentiate to diverse types of neurons.