T‐lymphocytes and Cytotoxic Astrocyte Blebs Correlate Across Autism Brains




Autism spectrum disorder (ASD) affects 1 in 59 children yet, except for rare genetic causes, the etiology in most ASD remains unknown. In the ASD brain, inflammatory cytokine and transcript profiling show increased expression of genes encoding mediators of the innate immune response. We evaluated post‐mortem brain tissue for adaptive immune cells and immune cell‐mediated cytotoxic damage that could drive this innate immune response in the ASD brain.


Standard neuropathology diagnostic methods including histology and immunohistochemistry were extended with automated image segmentation to quantify identified pathologic features in the postmortem brains.


We report multifocal perivascular lymphocytic cuffs contain increased numbers of lymphocytes in ~65% of ASD compared to control brains in males and females, across all ages, in most brain regions, and in white and grey matter, and leptomeninges. CD3+ T‐lymphocytes predominate over CD20+ B‐lymphocytes and CD8+ over CD4+ T‐lymphocytes in ASD brains. Importantly, the perivascular cuff lymphocytes numbers correlate to the quantity of astrocyte‐derived round membranous blebs. Membranous blebs form as a cytotoxic reaction to lymphocyte attack. Consistent with multifocal immune cell‐mediated injury at perivascular CSF‐brain barriers, a subset of white matter vessels have increased perivascular space (with jagged contours) and collagen in ASD compared to control brains. CSF‐brain barrier pathology is also evident at cerebral cortex pial and ventricular ependymal surfaces in ASD.


The findings suggest dysregulated cellular immunity damages astrocytes at foci along the CSF‐brain barrier in ASD.

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