Nuclear factor of activated T-cells 5 (NFAT5) activation and subsequent induction of the downstream osmolyte pathway has been shown in inflammatory myopathies. In this study, we tested these pathways in an in vitro cell model of muscle inflammation, studying NFAT5 and its molecular targets SLC6A12, SLC5A3, SLC6A6 and AKR1B1, using quantitative PCR, western blotting and immunofluorescence. In primary normal myotubes, both pro-inflammatory cytokines and increased salt concentrations were able to induce the osmolyte pathway.

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