The purpose of this study is to compare two methods of muscle segmentation to assess muscle volumes in human calf muscles. In this prospective cross-sectional study, we evaluated lower leg muscles of 18 healthy volunteers (9 females, mean age 27.6 ± 3.8 years) using muscle diffusion tensor imaging (DTI) and tractography. MRI was performed using a 3T MRI (Achieva 3T X, Philips medical Systems) and a 16 channel Philips Torso XL coil. The lower legs were separated into two FOV to improve shimming. For every FOV a sequence of T1w, T2w and diffusion weighted imaging were acquired.

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